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Description
Human TERF2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a Telomeric Repeat Binding Factor 2 (TERF2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Telomeric Repeat Binding Factor 2 (TERF2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Telomeric Repeat Binding Factor 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Telomere repeat binding factor 2 (TERF2), also known as TRF2 and TRBF2, is a protein encoded by the TERF2 gene. It is present at telomeres throughout the cell cycle. It is a component of the shelterin nucleoprotein complex and a secondary negative regulator of telomere length, playing a key role in telomere protection. It was first described in 1997 in the laboratory of Titia de Lange, who identified a DNA sequence similar to, but not identical to, TERF1 with respect to the Myb domain. De Lange isolated the new Myb-containing protein sequence and named it TERF2. The TERF2 protein has four domain classes that enable it to bind to other proteins in the shelterin protein complex and to specific types of DNA. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.6 ★★★★★
Based on 9 reviews
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Product Reviews
★★★★★ 3
Good size but pee leaks through :(
Size: X-Large, Style: 40 Count, Size: X-Large, Style: 40 Count
I have a 13-14 year old dog who is starting to have very frequent accidents in the house despite us taking her outside every couple of hours, she just drinks too much water which doesn’t mesh well with her weakening, old bladder. She tends to have an accident in two areas (the kitchen and right by the front door hallway) so we decided to buy some puppy pads to minimize the mess & aid in cleanup since clearly taking her outside every couple frequently isn’t helping reduce accidents. We chose these because of the size & price, which are both fantastic compared to others. It perfectly fits the two areas she tends to go so we only need to use one per area and to our surprise, she still remembers how to use puppy pads so she adapted quickly.
But unfortunately, these don’t hold all of her pee as they are very thin. I just cleaned up the kitchen puppy pad and saw moisture under the pee spot on the pad. So while it does dramatically reduce how much we need to clean up (we were using one paper towel roll every 3 days just for her accidents), we still need to wipe down the floor with pet accident cleaning spray & mop. It’s not that big of an issue but it is still a bit frustrating that they don’t hold fluid well.
So keep that in mind if you decide to order these, that you might still need to wipe down the floor under where your puppy/dog pees. We’ll be trying a different brand when we run out of these to see if that’s any better.
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Reviewed in the United States on February 18, 2026
★★★★★ 5
Great buy
Size: Giant, Style: 40 Count
These pads are great. Absorbent and leak resistant.
Very roomy for my chihuahua to turn in her inconsistent circles before and durable enough for her to "dig" after without ripping or bunching up.
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Reviewed in the United States on May 21, 2026
★★★★★ 5
Solid interactive automatic vibrating dog ball toy
Color: Blue, Color: Blue
Picked up this ITEHZTO interactive automatic vibrating dog ball for our pup and it’s a fun, engaging toy that keeps her entertained. It’s rechargeable with USB, motion-activated, and has a good vibrating/rolling action that gets her chasing and playing on her own. The durable build seems like it can handle some rough play, and it’s a nice size for indoor or outdoor use.
It lights up or vibrates when activated which adds extra excitement, especially during evening playtime. Battery life is decent and recharges quickly. Great for when she needs mental and physical stimulation without me having to throw a ball constantly.
Overall a solid interactive dog toy that does a great job keeping active dogs busy. Good value if your dog loves chasing moving/vibrating toys!
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Reviewed in the United States on April 30, 2026
★★★★★ 4
Built-in USB-C solves the power issue, but it is strictly for moderate chewers
Color: Blue
Got this to keep the high-energy puppy occupied indoors so the older dogs can actually get some peace and quiet. The main issue with most automated electronic pet toys is that they rely on expensive, hard-to-find button cell batteries that die after a single afternoon of use.
This unit utilizes a built-in 300mAh battery with a standard Type-C charging interface. That is a mandatory design feature if you actually want to run the toy daily without going broke replacing power cells. The internal motor generates enough erratic movement to keep a dog's attention, and it actually has the torque to navigate over standard tile, hardwood, and light carpets without instantly stalling out in a corner.
You need to completely understand the physical limitations of what you are unboxing here. The listing explicitly states this is not for heavy chewers, and you need to take that warning seriously. The outer shell is TPU, but the internal chassis housing the electronics is hard ABS plastic. If you hand this to an 80-pound working dog that aggressively shreds thick bones, they are going to crush the housing and destroy the internal motor assembly in about ten minutes.
At roughly 2.5 inches in diameter, it's basically the size of a standard tennis ball. If you use it as intended—as an interactive chasing toy for a puppy or a moderate chewer rather than a heavy-duty chew toy—it functions perfectly. It is a highly practical piece of indoor enrichment gear. Solid return on investment for burning off excess canine energy.
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Reviewed in the United States on May 29, 2026
★★★★★ 5
Pawsitively Stimulating🐾
Color: Blue
When my hooman brought this home, I thought it was fur their computer. After all, it has a plug and lights up. When they placed it on the floor, I was pawsomely surprised. Fur real, this little ball is wierd. It doesn't taste at all like my favorite tennis ball. I was a pawplexed that sometimes when I was finally brave enough to get it and hold it in my mouth, it would start jumping around. I think my hoomans finally found a toy that will keep me busy for a while. Mom is howling with excitement because it kept me interested for a long time. I hope I can sleep tonight. I see mom has plugged in my new toy to keep it "charged"- whatever that means....I never had a toy that came with its own leash before. Mom says she is gonna save the toy until other hoomans come over to keep me from being too ruff with them. You see the video, do I look a dog who could be too ruff?- Sincerely, the family dog, a bona-fido toy reviewer
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Reviewed in the United States on May 3, 2026